Open AccessIn vitro Plant Regeneration in Cleopatra (Citrus reshni Hort. ex Tan.) by Direct Organogenesis
Author(s) -
Uma Devi,
H. S. Rattanpal
Publication year - 2018
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v28i2.39683
Subject(s) - shoot , epicotyl , organogenesis , explant culture , biology , cleopatra , botany , murashige and skoog medium , horticulture , in vitro , biochemistry , gene
Maximum shoot regeneration (87.85%) and number of shoots per explant in the epicotyl segments having longitudinal cut in Citrus reshni var. Cleopatra were obtained on MT medium supplemented with BAP (2 mg/l) + NAA (0.2 mg/l). Maximum shoot length (5.10 cm), average leaf number per shoot (12), leaf length (2.12 cm) and width (1.5 cm) in Cleopatra were obtained on MT medium supplemented with BAP (2 mg/l) + NAA (0.2 mg/l). The maximum in vitro rooting (78.88 %) in the excised shoots occurred in liquid medium on MS supplemented with BAP (0.5 mg/l) + NAA (0.5 mg/l).
Plant Tissue Cult. & Biotech. 28(2): 251-260, 2018 (December)