Open AccessHigh Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.
Author(s) -
Tanjina Akhtar Banu,
Barna Goswami,
Shahina Akter,
Md. Ekramul Islam,
Tammana Tanjin,
Ahsan Habib,
Salim Khan
Publication year - 2017
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v27i2.35026
Subject(s) - explant culture , petiole (insect anatomy) , shoot , acclimatization , biology , botany , murashige and skoog medium , horticulture , in vitro , hymenoptera , biochemistry
An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1 ± 1.96. For petiole and leaf explants best response was observed on MS supplemented with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. Petiole explants produced highest mean number of shoots/explant (22.9 ± 1.728) among the three explants when the explants were cultured on MS with 2.0 mg/l BAP, 1 mg/l IAA and 0.5 mg/l Kn. The highest frequency of root induction (100%) and mean number of roots/plantlets (11.75) were obtained on MS. The rooted plantlets were transferred for hardening following acclimatization and finally were successfully established in the field.Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)