Open AccessHigh Frequency Regeneration of Plantlets from Immature Male Floral Explants of Musa paradisica cv. Puttabale - AB Genome
Author(s) -
Kaushalendra Kumar,
V. Krishna,
Venkatesh Venkatesh,
Kannampalli Pradeep
Publication year - 2011
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v21i2.10243
Subject(s) - explant culture , callus , shoot , biology , agar , botany , activated charcoal , murashige and skoog medium , sucrose , horticulture , in vitro , chemistry , genetics , organic chemistry , adsorption , bacteria , biochemistry
High frequency in vitro regeneration for mass multiplication from immature male floral explants of Musa paradisica cv. Puttabale on MS supplemented with adenine sulfate (160 mg/l), tyrosine (100 mg/l), sucrose (40 g/l) and gelled with 0.8 g/l agar was attempted. For callus induction the combinations of 2, 4-D and BAP were tested at 1.0 - 10.0 mg/l and 0.5 - 5.0 mg/l, respectively. For shoot bud formation combinations of BAP and TDZ were also tested at 1.0 - 5.0 mg/l and 0.1 - 0.5 mg/l, respectively. Luxuriant proliferation and high frequency induction (97.0%) of callus was noticed from the accessory floral part of the explant at 7.0 mg/l 2, 4-D and 1.0 mg/l BAP, later it preceded towards the gynoecium. Interaction of BAP (2.0 5.0 mg/l) and TDZ (0.2 - 0.5 mg/l) would provoke high frequency shoot bud differentiation from the floral calli and a mean of 29.40 ± 6.10 shootlets per callus was obtained at 4 mg/l BAP and 0.4 mg/l TDZ. Rooting of the microshoots was achieved on MS containing 0.6 mg/l NAA and 0.2% activated charcoal. Key words: Musa pardisica, Puttabale, Regeneration, Male floral explants. D. O. I. 10.3329/ptcb.v21i2.10243 Plant Tissue Cult. & Biotech. 21(2): 199-205, 2011 (December)