Open AccessMicropropagation of Marsdenia brunoniana Wight & Arn. - A Rare Antidiabetic Plant
Author(s) -
A. Ugraiah,
S. Karuppusamy,
T. Pullaiah
Publication year - 2012
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v21i1.9613
Subject(s) - shoot , explant culture , micropropagation , plant growth , biology , rice plant , horticulture , botany , in vitro , biochemistry
Shoot multiplication of Marsdenia brunoniana was obtained from the nodal explants of mature plants using MS with different concentrations and combinations of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS fortified with 1.0 mg/l BAP. The highest degree of shoot profilieration was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening, survival rate was 90%. Key words: Marsdenia brunoniana, Antidiabetic plant, Micropropagation, Conservation D. O. I. 10.3329/ptcb.v21i1.9613 Plant Tissue Cult. & Biotech. 21(1): 89-93, 2011 (June) - Short communication