Open AccessEffect of Picloram on Somatic Embryogenesis from Leaf-sheath Explants in Diploid Musa acuminata cv. Njalipoovan
Author(s) -
P.D. Smitha,
A. Sreekumaran Nair
Publication year - 2012
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v21i1.9612
Subject(s) - picloram , somatic embryogenesis , biology , explant culture , callus , musa acuminata , botany , ploidy , shoot , embryo , murashige and skoog medium , horticulture , embryogenesis , microbiology and biotechnology , in vitro , biochemistry , gene
Embryogenic callus was obtained from leaf sheath explants on MS supplemented with 16.56 μM picloram in Musa acuminata (diploid) cv. Njalipoovan (AB). Exogenous application of ascorbic acid on somatic embryogenesis at optimal level accelerated the conversion rate of the embryos. Shoot and root developed from the embryos on MS incubated in dark. Further growth of plantlets obtained on half strength of MS under light conditions. Regenerated plantlets showed 100% survival. Key words: Musa acuminata cv. Njalipoovan, Picloram, Somatic embryogenesis D. O. I. 10.3329/ptcb.v21i1.9612 Plant Tissue Cult. & Biotech. 21(1): 83-87, 2011 (June) - Short communication