An Improved Plant Regeneration of Vanilla planifolia Andrews | Zendy

Boon Chin Tan | Zendy; Chiew Foan Chin | Zendy; P. G. Alderson | Zendy

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An Improved Plant Regeneration of Vanilla planifolia Andrews

Author(s) -

Boon Chin Tan,

Chiew Foan Chin,

P. G. Alderson

Publication year - 2012

Publication title -

plant tissue culture and biotechnology

Language(s) - English

Resource type - Journals

eISSN - 1818-8745

pISSN - 1817-3721

DOI - 10.3329/ptcb.v21i1.9560

Subject(s) - shoot , explant culture , acclimatization , biology , botany , horticulture , regeneration (biology) , tissue culture , in vitro , biochemistry , microbiology and biotechnology

Multiple shoots of Vanilla planifolia were induced from nodal explants under influence of different concentrations of plant growth regulators and coconut water (CW). A comparison of shoot regeneration between semi solid and liquid culture media was also investigated. Ninety seven per cent of the explants produced a mean number of 9.6 shoots with a mean shoot length of 4.70 cm when cultured on liquid MS supplemented with 1.0 mg/l BAP in combination of 15% CW. Shoots generated were rooted with frequency of 93% on MS supplemented with 1.0 mg/l NAA with a mean number of 2.9 roots per shoot and mean length of 4.0 cm within four weeks. The plantlets were transferred to sand : compost mixture (1 : 2) with 85.0% survival rate recorded after four weeks of acclimation. Key words: Regeneration, coconut water, nodal culture, vanilla D.O.I. 10.3329/ptcb.v21i1.9560 Plant Tissue Cult. & Biotech. 21(1): 27-33, 2011 (June)

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