Open AccessRapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant
Author(s) -
Nabarun Ghosh,
Don W. Smith,
Anath Bandhu Das,
Amlan Chatterjee
Publication year - 2010
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v20i1.5966
Subject(s) - explant culture , biology , shoot , callus , botany , tissue culture , horticulture , leaflet (botany) , micropropagation , in vitro , biochemistry
Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically. Key words: Albizia falcataria, Leaf explant, Clonal propagation, Cytology D.O.I. 10.3329/ptcb.v20i1.5966 Plant Tissue Cult. & Biotech. 20(1): 63-72, 2010 (June)