Open AccessEfficient Plant Regeneration from Shoot Apices of Pearl Millet (Pennisetum americanum (L.) Leeke)
Author(s) -
Y. Varalaxmi,
A. Vijayalakshmi,
Keshav Kumar,
T. Vijayalakshmi,
M. Maheswari
Publication year - 2010
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v20i1.5964
Subject(s) - pennisetum , shoot , biology , explant culture , pearl , regeneration (biology) , plant tissue , botany , horticulture , poaceae , agronomy , in vitro , biochemistry , philosophy , theology , microbiology and biotechnology
An efficient and rapid regeneration protocol was developed from shoot tip explants of Pennisetum americanum (L.) Leeke variety WC-75. MS supplemented with low concentration of 2,4-D and Kn was most effective in producing embryogenic calli. Maximum regeneration potential of 40 shoots per calli were obtained when transferred to regeneration medium containing 1.0 mg/l Kn. Shoots developed were efficiently rooted within 15 days on the medium containing NAA. Over 90% of rooted plants were fertile after transfer to a net house. Key words: Pearl millet, Efficient, plant regeneration, Shoot apices D.O.I. 10.3329/ptcb.v20i1.5964 Plant Tissue Cult. & Biotech. 20(1): 47-53, 2010 (June)