Open AccessHistological Marker to Differentiate Organogenic Calli from Non Organogenic Calli of Gloriosa superba L.
Author(s) -
Manju Madhavan,
Joy P. Joseph
Publication year - 2010
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v20i1.5957
Subject(s) - callus , biology , organogenesis , botany , xylem , gene , biochemistry
A histological study of the indirect organogenesis from internodal cultures of Gloriosa superba L. showed that the callus initiated from the subepidermal cells. The organogenic and non organogenic callii are the result of hormonal variation in the medium. In non organogenic callus cells redifferentiated into xylem elements forming clusters of nest like structures. In organogenic callus, cells redifferen-tiated into nodules of meristemoids which further differentiated into shoot apical meristem. Keywords: Histological marker, Gloriosa superba, Organogenic calli D.O.I. 10.3329/ptcb.v20i1.5957 Plant Tissue Cult. & Biotech. 20(1): 1-5, 2010 (June)