Open AccessIn vitro Plant Regeneration from Seedling-derived Explants of two Cultivars of White Jute (Corchorus capsularies L.)
Author(s) -
RH Sarker,
Bivas Kumar Sarkar,
M. I. Hoque
Publication year - 2010
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v19i2.5437
Subject(s) - cotyledon , explant culture , biology , shoot , seedling , petiole (insect anatomy) , botany , acclimatization , murashige and skoog medium , cultivar , regeneration (biology) , horticulture , in vitro , hymenoptera , biochemistry , microbiology and biotechnology
An in vitro regeneration system for two varieties of white jute (Corchorus capsularis L.) namely, BJC-7370 and BJC-83 was developed. The regeneration protocol was based upon direct organogenesis from seedling-derived explants such as cotyledon with petioles, cotyledonary node. Shoot regeneration was achieved from cotyledon with petiole and cotyledonary node through use of MS supplemented with 0.5 mg/l BAP and 1.0 mg/l IAA for BJC-7370 while for BJC-83 shoot initiation was obtained on 1.25 mg/l BAP and 0.25 mg/l NAA using the same explants. Elongation of shoots was achieved on MS containing 0.2 mg/l BAP for the two said jute varieties. Regenerated excised shoots developed effective in vitro root system on half strength of MS supplemented with 0.3 mg/l IBA for both the varieties. The in vitro grown plantlets were transferred to soil for acclimation. These plants grew up to maturity, flowered and produced seeds identical to the control plants. Key words: Corchorus capsularies, In vitro plant regeneration, seedling explants D.O.I. 10.3329/ptcb.v19i2.5437 Plant Tissue Cult. & Biotech. 19(2): 199-206, 2009 (December)