Open AccessClonal Propagation Through Nodal Explant Culture of Boerhaavia diffusa L. - A Rare Medicinal Plant
Author(s) -
Animesh Biswas,
M. A. Bari,
Mohashweta Roy,
S. K. Bhadra
Publication year - 1970
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v19i1.4917
Subject(s) - explant culture , acclimatization , shoot , biology , tissue culture , horticulture , botany , micropropagation , in vitro , murashige and skoog medium , plant growth , biochemistry
Multiple shoots were induced on MS fortified with 2.0 mg/l BAP and 0.2 mg/l NAA within 30 days of culture. Maximum (93%) explants produced multiple shoots with an average 12 shoots per culture after two successive subcultures at 14 days interval in the same medium. Cent per cent rooting was achieved in half strength of MS supplemented with 1.0 mg/l IBA. Following 21 days in vitro rooting period and seven days of ex vitro acclimatization, plantlets were successfully established in natural condition. The survival rate of regenerated plants was 90 per cent. Key words: Clonal propagation, Boerhaavia diffusa, Rare medicinal plant, Nodal culture D.O.I. 10.3329/ptcb.v19i1.4917 Plant Tissue Cult. & Biotech. 19(1): 53-59, 2009 (June)