Open AccessAgrobacterium-mediated Transformation and Constitutive Expression of PgNHX1 from Pennisetum glaucum L. in Oryza sativa L. cv. Binnatoa
Author(s) -
Saiful Islam,
Rumana S Tammi,
Sneh L. SinglaPareek,
Zeba I. Seraj
Publication year - 1970
Publication title -
plant tissue culture and biotechnology
Language(s) - English
Resource type - Journals
eISSN - 1818-8745
pISSN - 1817-3721
DOI - 10.3329/ptcb.v19i1.4912
Subject(s) - pennisetum , transformation (genetics) , biology , oryza sativa , transgene , agrobacterium , salinity , genetically modified crops , gene , southern blot , botany , genetically modified rice , gene expression , horticulture , microbiology and biotechnology , genetics , ecology
In an effort to improve salinity tolerance in rice, the Pennisetum glaucum vacuolar Na+/H+ antiporter gene (PgNHX1) was transformed and expressed in Bangla-deshi rice Binnatoa under the constitutive promoter CaMV35S. Transgenic status of the plant was confirmed by PCR and Southern blot hybridization. RT-PCR and Western dot-blot analyses were performed to validate the expression of the transgene at RNA and protein levels, respectively. At 160 mM (ECe of 16 dS/m) NaCl stress the transgenic seedlings showed enhanced tolerance compared to controls. Key words: Salinity tolerance, PgNHX1, CaMV35S, Binnatoa D.O.I.10.3329/ptcb.v19i1.4912 Plant Tissue Cult. & Biotech. 19(1): 25-33, 2009 (June)