Open AccessIn vitro antioxidant activity of the methanolic extract of leaves of a hill tract plant Begonia roxburghii
Author(s) -
Taslima Akter,
Afra Nawar,
Nur Alam,
Rafiquzzaman
Publication year - 2021
Publication title -
jahangirnagar university journal of biological sciences
Language(s) - English
Resource type - Journals
ISSN - 2306-0263
DOI - 10.3329/jujbs.v9i1-2.53709
Subject(s) - ascorbic acid , gallic acid , chemistry , dpph , flavonoid , quercetin , phytochemical , antioxidant , food science , traditional medicine , chromatography , botany , biochemistry , biology , medicine
The study was designed to investigate the antioxidant activities of the methanolic extract of leaves of Begonia roxburghii by five different in vitro methods namely Folin-Ciocalteu, total flavonoid content, phosphomolybdenum, total reducing power determination, and DPPH scavenging activity. Phenol content (Gallic acid equivalent) was found as 180.40±0.03 mg/g of the dry sample by the Folin-Ciocalteu method. Flavonoid assay method revealed the content of flavonoids (Quercetin equivalent) as 60.43±0.27 mg/g of the dry sample. Total antioxidant capacity of the extract was observed 243.43±0.11 mg/g ( ascorbic acid equivalent) by phosphomolybdenum method. Free radical scavenging activity (measured by the DPPH test) of the methanolic extract was found 158.36±0.22 μg/mL whereas ascorbic acid, the standard, showed 19.53±0.04 μg/mL. The methanolic extract of Begonia roxburghii leaves exhibited positive and linear dose dependent reducing power activity and its activity was comparable to the standard ascorbic acid for a given dose. Phytochemical screening of the extract showed positive results for alkaloids, carbohydrates, flavonoids, glycosides, saponins, steroids, and tannins, which demonstrate definite pharmacological actions on human body. The extract showed negative results for the steroids.
Jahangirnagar University J. Biol. Sci. 9(1 & 2): 79-89, 2020 (June & December)