Open AccessRepeated batch fermentation for protease production using biofilm fermentation of Bacillus sp.
Author(s) -
Ripa Moni,
M. Salahuddin,
A. H. Khan,
Umme Salma Zohora,
Mohammad Shahedur Rahman
Publication year - 2018
Publication title -
jahangirnagar university journal of biological sciences
Language(s) - English
Resource type - Journals
ISSN - 2306-0263
DOI - 10.3329/jujbs.v6i2.36588
Subject(s) - bacillus subtilis , fermentation , protease , biofilm , food science , enzyme , microbiology and biotechnology , chemistry , bacteria , biology , biochemistry , genetics
Repeated batch fermentation is a stable, economically viable and environment friendly fermentation system. Biofilm of Bacillus subtilis was investigated for repeated batch fermentation for the production of protease enzyme. It was found that complete replacement of spent medium with fresh medium supplementation in biofilm produced similar amount of protease that is about 225 U/ml of enzyme in successive batches. Moreover, unlike some solid carriers the EPS layer does not entrap much enzyme inside it. Therefore, instead of using immobilized cells, biofilm of B. subtilis can be a good candidate for protease enzyme production using repeated batch fermentation.Jahangirnagar University J. Biol. Sci. 6(2): 29-38, 2017 (December)