Open AccessNeuroprotective Properties of Nigella sativa (L.) Seeds Extract in Sprague Dawley Rats models
Author(s) -
Zahir Uddin Mohammed Babar,
Abul Kalam Azad,
Wan Mohd. Azizi Wan Sulaiman,
Jalal Uddin,
Zubair Khalid Labu
Publication year - 2018
Publication title -
the dhaka university journal of pharmaceutical sciences
Language(s) - English
Resource type - Journals
eISSN - 1816-1839
pISSN - 1816-1820
DOI - 10.3329/dujps.v17i1.37127
Subject(s) - nigella sativa , neuroprotection , glial fibrillary acidic protein , neurodegeneration , hippocampus , pharmacology , hippocampal formation , antioxidant , neuroinflammation , chemistry , traditional medicine , medicine , biochemistry , inflammation , immunohistochemistry , disease
Neuroinflammation has been closely linked to neurodegeneration that leads to dysfunction of memory and learning where glial fibrillary acidic protein (GFAP) plays important role positively within hippocampus. In experimental neurodegeneration achieved by two-vessel occlusion (2VO) intervention, Nigella sativa (L.) seeds extract (50 mg/kg/day orally) has shown to have anti-neuroinflammatory and antioxidant properties. The brain hippocampal tissues were humanely collected at the end of 10th treatment week and preserved in Allprotect™ reagent at –80°C. Total RNA was extracted and purified by phenol/chloroform method using kits and reverse transcribed into cDNA and relatively quantified as per ΔΔCq. The NSSE treatment showed significantly (p 0.5) different to that of healthy control (HC). Prolonged or daily treatment with NSSE (Nigella sativa (L.) seeds extract) may provide moderate anti-neuroprotective activity within hippocampus.Dhaka Univ. J. Pharm. Sci. 17(1): 113-121, 2018 (June)