Open AccessStudies of Physico-chemical Properties and Cytotoxicity of Fruits of Syzygium jambos L. against HeLa and Vero Cell Lines
Author(s) -
Fajilatun Nesa,
Mohammad Shoeb,
Md. Samiul Islam,
Nazrul Islam
Publication year - 2021
Publication title -
bangladesh pharmaceutical journal
Language(s) - English
Resource type - Journals
eISSN - 2408-8463
pISSN - 0301-4606
DOI - 10.3329/bpj.v24i2.54709
Subject(s) - hela , gallic acid , dichloromethane , traditional medicine , chemistry , ethyl acetate , hexane , cytotoxicity , food science , botany , chromatography , biology , organic chemistry , solvent , antioxidant , biochemistry , medicine , cell , in vitro
Syzygium jambos L. belonging to the family Myrtaceae has a long history of using as a dietary fruit and folklore medicine. Investigation of this fruit was carried out to evaluate the different chemical properties and biological activities. The moisture and ash content of the fruit sample were calculated as 86.88 ± 0.61% and 0.29±0.02%, respectively. Dried powder of the fruit was extracted successively with nhexane, dichloromethane (DCM) and methanol (MeOH). UV-Visible and FT-IR spectral analyses confirmed the presence of unsaturated carboxylic acid in n-hexane, unsaturated ester in DCM and a diketone in MeOH extracts. Cytotoxicity assay of different extracts was carried out against HeLa and Vero cell lines and no extract was found to be cytotoxic. Total phenolic contents of the n-hexane, DCM and MeOH extracts were 15.39 ± 0.24, 31.32 ± 0.25 and 42.19 ± 0.16 mg gallic acid equivalent per gram of dry extract, respectively and total flavonoid content of n-hexane, DCM and MeOH extracts were 6.52 ± 0.18, 15.55 ± 0.16 and 8.36 ± 0.16 mg quercetin equivalent per gram of dry extract, respectively. This study establishes that S. jambos fruit can be a potential source of natural antioxidants.
Bangladesh Pharmaceutical Journal 24(2): 111-116, 2021