Open AccessCHARACTERIZATION OF HUMAN GRANULOCYTIC EHRLICHIOSIS IN EXPERIMENTAL INFECTED HL-60 CELLS WITH ANAPLASMA PHAGOCYTOPHILUM USING NESTED PCR AND A. PHAGOCYTOPHILUM MAJOR SURFACE PROTEIN-2 MONOCLONAL ANTIBODY
Author(s) -
MS Rahman,
Park Jh,
Chae Js
Publication year - 2012
Publication title -
bangladesh journal of veterinary medicine
Language(s) - English
Resource type - Journals
eISSN - 2308-0922
pISSN - 1729-7893
DOI - 10.3329/bjvm.v3i2.11400
Subject(s) - anaplasma phagocytophilum , nested polymerase chain reaction , monoclonal antibody , ehrlichiosis , biology , virology , surface protein , western blot , anaplasma , antibody , microbiology and biotechnology , polymerase chain reaction , gene , immunology , tick , genetics , borrelia burgdorferi
The study was carried out to characterize the human granulocytic ehrlichiosis in experimental infected HL-60 cells with Anaplasma phagocytophilum using nested PCR and A. phagocytophilum major surface protein-2 monoclonal antibody. The nested PCR revealed only one band of 926 base pair DNA from A. phagocytophilum infected HL-60 cells. The western blot revealed several bands with a dominant 44-kDa. There were intense band of 100- and 160-kDa bands. The 44-kDa component was at least 10 times more abundant than the 100- and 160-kDa bands. In conclusion, the nested PCR would be a valuable tool for the characterization of human granulocytic ehrlichiosis.