Open Access<i>In vitro</i> antibacterial activity and phytochemical analysis of hexane extract of <i>Vicia sativa</i>
Author(s) -
Mohammad Saleem,
Mahrukh Karim,
Muhammad Imran Qadir,
Bilal Ahmed,
Maria Rafiq,
Bashir Ahmad
Publication year - 2014
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v9i2.17859
Subject(s) - phytochemical , antibacterial activity , traditional medicine , chemistry , stigmasterol , vicia sativa , staphylococcus aureus , nigella sativa , bacteria , microbiology and biotechnology , biology , chromatography , botany , medicine , genetics
Vicia sativa is traditionally used medicinal plant in skin infections, asthma, bronchitis, urinary diseases and also used as antiseptic, anti-poison, aphrodisiac, anti rheumatic and antipyretic. In the present study n-hexane extract of V. sativa was evaluated for the antibacterial activity against pathogenic bacteria Staphylococcus aureus, Bacillus atrophaeus, Escherichia coli and S. epidermidis by disc diffusion method. Minimum inhibitory concentration of the n-hexane extract against all bacteria was determined by broth dilution method. Preliminary phytochemical analysis and HPLC analysis showed the presence of a number of bioactive constituents which exhibits antibacterial activity. So the current study showed that V. sativa possesses the significant antibacterial activity