Open AccessAlpha glucosidase inhibitory effect and enzyme kinetics of coastal medicinal plants
Author(s) -
Gurudeeban Selvaraj,
K. Satyavani,
T. Ramanathan
Publication year - 2012
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v7i3.11499
Subject(s) - alpha glucosidase , uncompetitive inhibitor , citrullus colocynthis , enzyme , enzyme assay , postprandial , chemistry , inhibitory postsynaptic potential , non competitive inhibition , enzyme kinetics , biochemistry , traditional medicine , biology , botany , medicine , microbiology and biotechnology , active site , insulin , neuroscience
Numerous metabolic disorders and diseases associated with the glucosidase enzyme activity. Therefore, in the present study we evaluated alpha glucosidase inhibitory effect of coastal medicinal plants on Saccharomyces cerevisiae α-glucosidase to control postprandial hyperglycemia. The n-hexane extracts of study plants screened for α-glucosidase inhibitory activity using yeast glucosidase. The enzyme kinetics studied Line weaver burk plot method. Among the ten plants Citrullus colocynthis, Aegle marmelos and Ipomoea pes-caprae exhibited potent α-glucosidase inhibitory activity, 85.9 ±0.10, 72.23 ± 0.30 and 67.9 ± 0.11 respectively. From the enzyme kinetics assay potent plants contain uncompetitive inhibitors of alpha glucosidase. C. colocynthis, A. marmelos and Ipomoea pes-caprae have potential alpha-glucosidase inhibitors that can exploited for its used to treat diabetes