Open Access<i>In vitro </i>anti-viral effect of fructopyrano-(1→4)-glucopyranose from <i>Radix isatidis</i> on infuenza virus A
Author(s) -
Qin Sun,
Pi Xian Shui,
Han Bin Li,
Yang Si,
Minghua Liu,
Shi Jing Zhang
Publication year - 2012
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v7i3.11228
Subject(s) - in vitro , virus , hemagglutinin (influenza) , neuraminidase , influenza a virus , radix (gastropod) , virology , biology , chemistry , biochemistry , botany
To investigate the in vitro anti-viral effect of fructopyrano-(1→4)-glucopyranose (FG) from traditional Chinese medicine Radix isatidis, dog kidney cells (MDCK) were with treated with either FG and then with virus (Group A); virus and then with FG (Group B); or FG and virus simultaneous-ly (Group C). Results showed the in vitro cell protection rate and virus inhibition rate increased significantly with the increase of FG concentration when compared with cells without FG treatment (p<0.01). The anti-viral effect was the best in Group C and that in Group A was superior to that in Group B. FG could exert significant inhibited effect on neuraminidase activity with the IC50 of 0.07 mmol/mL and on hemagglutinin of avian influenza virus with minimum inhibitory concentration of 3.13 mg/mL. Our findings indicate FG can confer obvious antiviral effects on influenza A virus in vitro