Open AccessAnti-oxidant, DNA-damage protection and anti-cancer properties of n-butanol extract of the endemic Perralderia coronopifolia
Author(s) -
Khadidja Bekhouche,
Tevfik Özen,
Sara Boussaha,
Serkan Koldaş,
Semiha Yenigün,
Somia Lassed,
İbrahim Demirtaş,
Fadila Benayache,
Samir Benayache,
Djamila Zama
Publication year - 2018
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v13i1.34255
Subject(s) - hydrogen peroxide , flavonoid , dna damage , chemistry , hela , microbiology and biotechnology , dna , dpph , reactive oxygen species , oxidative phosphorylation , biochemistry , in vitro , antioxidant , biology
This study was designed to evaluate in vitro the total phenolic, flavonoid content, anti-oxidant activity, oxidative DNA-damage protection and anti-cancer activity of n-butanol extract from Perralderia coronopifolia, endemic plant. DNA protection capacity was performed using 46966 plasmid DNA against UV-photolysis of H 2 O 2 -induced oxidative damage. The anti-prolifer-ative effects of extract were performed on HeLa and C6 cells. Experimental results showed that extract had convenient number of phenolic and flavonoids. Furthermore, it provided strong anti-oxidant, reducing power, hydrogen peroxide and higher DPPH· scavenging ability with IC 50 = 7.02 ± 0.02 µg/mL. As it shown an oxidative plasmid DNA-damage inhibition against UV-photolysis of H 2 O 2 , an anti-cancer activity athigher concentrations in cells. The data obtained that P. coronopifolia extract could be useful in human protection against infection and degenerative illness.