Open Accessα-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots
Author(s) -
Songül Karakaya,
Hikmet Özbek,
Sefa Gözcü,
Zühal Güvenalp,
Hafize Yuca,
Hayri̇ Duman,
Cavit Kazaz,
Ceyda Sibel Kılıç
Publication year - 2018
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v13i1.33668
Subject(s) - imperatorin , bergapten , chemistry , umbelliferone , amylase , stigmasterol , alpha glucosidase , biochemistry , enzyme , high performance liquid chromatography , chromatography , coumarin , psoralen , dna , organic chemistry
Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC 50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC 50 = 4.9 mg/mL). Grandivitinol (IC 50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species. Video Clip of Methodology : 8 min 26 sec: Full Screen Alternate