Open AccessIn vitro antifungal, anti-oxidant and HPLC analysis of the extracts of Physalis philadelphica
Author(s) -
Wajid Khan,
Jehan Bakht,
Bilal Muhammad Khan
Publication year - 2017
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v12i3.31965
Subject(s) - gallic acid , high performance liquid chromatography , rhizopus oryzae , chemistry , aspergillus flavus , ethyl acetate , chromatography , methanol , minimum inhibitory concentration , physalis , traditional medicine , in vitro , antioxidant , food science , botany , biochemistry , biology , organic chemistry , fermentation , medicine
The different parts of Physalis philadelphica were evaluated for the antifungal, anti-oxidant and HPLC assay. The methanol extract of leaf showed inhibitory activity against Pencillium digitatum (58% ZI), Aspergillus flavus (95% ZI) and Rhizopus oryzae (96% ZI) at a concentration of 400 ppm. Strong anti-oxidant activities were found in different parts of the plant. The effects of different extraction solvents on the anti-oxidant activity and antifungal activity of the medicinal extracts were observed. The methanol extract from the leaf, stem, fruit and water fractions from the calyx exhibited strong anti-oxidant activity of 94, 89, 88 and 93% respectively. The ethyl acetate extract was found as a best solvent for the recovery of gallic acid in HPLC assay. No correlation was observed between the gallic acid and anti-oxidant capacity of the extracts. Video Clip of Methodology : 11 min 13 sec: Full Screen Alternate