Open AccessPhytochemical studies and antibacterial activity of the aerial parts of Physospermum verticillatum
Author(s) -
Ibtissem Boulacel,
Lakhdar Djarri,
Salima Azzouzi,
Kamel Medjroubi,
İbrahim Demirtaş,
Hocine Laouer,
Salah Akkal
Publication year - 2017
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v12i2.29668
Subject(s) - pentadecanoic acid , triterpene , chemistry , ethyl acetate , phytochemical , chromatography , sterol , carbon 13 nmr , fatty acid , stereochemistry , organic chemistry , biochemistry , cholesterol , medicine , alternative medicine , pathology
In this study, we focused on the isolation and identification of the main compounds, triterpene, sterol, phenolic and fatty acid from the aerial parts of Physospermum verticillatum, which has not been previously reported. Dichloromethane, ethyl acetate and n-butanol extracts led to the isolation of ß-amyrin (1), 3,11-dihydroxy-12-oleanene (2), pentadecanoic acid (3), spina-sterol (4), spinasterol-3-O-ß-D-glucopyranoside (5) and saikochromic acid (6). These compounds were determined by means of the combined systems with high resolution tandem mass spectrometry (HPLC-TOF/MS), 1D NMR ( 1 H NMR, 13 C NMR) and 2D NMR(COSY, HSQC and HMBC). Further, the extracts demonstrated significant antibacterial activity.