Open AccessChemical derivatization of pharmaceutical samples prior to Gas-Chromatography and Mass-Spectrometry analysis
Author(s) -
Vivek K. Bajpai,
Na-Hyung Kim,
Kang-Min Kim
Publication year - 2016
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v11i4.28588
Subject(s) - derivatization , chromatography , mass spectrometry , chemistry , gas chromatography–mass spectrometry , gas chromatography , elution , volatility (finance) , financial economics , economics
Derivatization is the process by which a compound is chemically changed, that has properties more amenable to a particular analytical method. Some samples analyzed by Gas-chromatography and Mass-spectrometry (GC-MS) require derivatization in order to make them suitable for analysis. Especially the compounds that have poor volatility, poor thermal stability, or that can be adsorbed in the injector will exhibit non-reproducible peak areas, heights, and shapes. Hence, this study focused on step-by-step visual demonstration of chemical derivatization of pharmaceutical samples prior to GC-MS analysis for their better elution and detection.