Open AccessEvaluation of the anti-cancer potential of <i>Mahonia aquifolium</i> extracts via apoptosis and anti-angiogenesis
Author(s) -
Ana Damjanović,
Gordana Zdunić,
Katarina Šavikin,
Boris Mandić,
Milka Jadranin,
Ivana Z. Matić,
Tatjana Stanojković
Publication year - 2016
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v11i3.27103
Subject(s) - apoptosis , angiogenesis , cell cycle , microbiology and biotechnology , mtt assay , hela , chemistry , cytotoxicity , flow cytometry , cancer cell , cell growth , cell culture , cell , in vitro , biology , cancer research , biochemistry , cancer , genetics
The cytotoxicity of Mahonia aquifolium ethanol and water extracts was examined using MTT test. The morphological changes were analyzed by fluorescence microscopy. Cell cycle distribution and possible activation of caspase-dependent pathway of cell death were assessed by flow cytometry. The effects of ethanol and water extracts on migration of endothelial EA.hy926 cells were analyzed by in vitro scratch assay and inhibition of angiogenesis was detected using tube formation assay. Both extracts demonstrated cytotoxic effects on cancer cell lines with very high selectivity. Morphological evaluation indicated apoptosis. These results were confirmed with cell cycle analysis, where there was accumulation of cancer cells in the subG1 phase. Ethanol and water extracts induced a caspase-dependent apoptosis in HeLa cells through activation of caspase-3 and caspase-8. Both extracts showed the ability to inbibit the migration of EA.hy926 cells and initial steps of angiogenesis. In addition, ethanol extract exerted significant anti-angiogenic effect