Open AccessInhibitory effect of <i>Sphagnum palustre</i> extract and its bioactive compounds on aromatase activity
Author(s) -
Hee Jeong Eom,
Yong Joo Park,
Hee Rae Kang,
Ha Ryong Kim,
In Jae Bang,
Hyun Bong Park,
Kyu Hyuck Chung,
KiHyun Kim
Publication year - 2016
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v11i3.26776
Subject(s) - aromatase , medicine , endocrinology , chemistry , ic50 , biology , biochemistry , in vitro , cancer , breast cancer
Sphagnum palustre (a moss) has been traditionally used in Korea for the cure of several diseases such as cardiac pain and stroke. In this research, the inhibitory effect of S. palustre on aromatase (cytochrome P450 19, CYP19) activity was studied. [1β-3H] androstenedione was used as a substrate and incubated with S. palustre extract and recombinant human CYP19 in the presence of NADPH. S. palustre extract inhibited aromatase in a concentration-dependent manner (IC50 value: 36.4 ± 8.1 µg/mL). To elucidate the major compounds responsible for the aromatase inhibitory effects of S. palustre extract, nine compounds were isolated from the extract and tested for their inhibition of aromatase activity. Compounds 1, 6, and 7 displayed aromatase inhibition, while the inhibition by the other compounds was negligible