Open AccessIsolation of flavonoids from onion skins and their effects on K562 cell viability
Author(s) -
Shi Guo-qing,
Jing Yang,
Jiang Liu,
Sheng-Nan Liu,
Han-Xue Song,
Zhao Wang,
Yanqi Liu
Publication year - 2016
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v11i0.26419
Subject(s) - isorhamnetin , rutin , kaempferol , quercetin , chemistry , apigenin , flavonoid , column chromatography , biochemistry , chromatography , antioxidant
To investigate the anti-proliferative activity of flavonoids from onion skins, extraction by 50% ethanol (v/v), soxhlet polar fractionation, pH gradient separation, thin-layer chromatography, and recrystallization methods were used to isolate and purify flavonoids from dry onion skins. Anti-proliferative activities of some flavonoids obtained on leukemia K562 cell line were deter-mined by MTT assay. Results showed that flavonoids of onion skins were mainly in form of quercetin, kaempferol, isorhamnetin, apigenin-7-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, kaempferol-7-O-β-D-glucopyranoside and rutin. Quercetin and kaempferol decreased K562 cell viability, and quercetin had stronger effect. However, isorhamnetin and rutin exhibited certain proliferation-promoting effects. It suggests that ortho hydroxyl groups on B ring of onion flavonoids might be the key structural elements of their cytotoxic effects on K562 cells, and hydroxyl groups in position 3 or carbonyl groups in position 4 might be one of the structural effect elements.