Open AccessAnticancer and anti-angiogenesis effect of the leaf extract of Sargassum wightii against osteosarcoma cancer cells
Author(s) -
Qi-Long Yu,
Zhao-Chun Tang,
Jinge Li,
Guoliang Wang,
Pei-Jun Wang,
Zhaobao Pan
Publication year - 2015
Publication title -
bangladesh journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.385
H-Index - 23
eISSN - 1991-0088
pISSN - 1991-007X
DOI - 10.3329/bjp.v10i2.22473
Subject(s) - angiogenesis , umbilical vein , vascular endothelial growth factor , mtt assay , in vivo , viability assay , sargassum , chemistry , reactive oxygen species , human umbilical vein endothelial cell , traditional medicine , flavonoid , biology , microbiology and biotechnology , pharmacology , in vitro , botany , biochemistry , vegf receptors , cancer research , medicine , antioxidant , algae
This study was aimed to evaluate the chemical composition of ethanolic leaf extract of a marine macro-alga Sargassum wightii (ELESW) followed by its vascular endothelial growth factor (VEGF) induced anti-angiogenic effect against human umbilical vein endothelial cells (HUVECs). The effect of ELESW on cell viability and its potential against in vivo angiogenesis was assessed by using the MTT and CAM assay respectively. The phenolic, flavonoid, polysaccharides, and protein content of ELESW were found to be 56.0 ± 4.1 mg GAE/g, 26.3 ± 3.6 mg QE/g, 284.2 ± 6.2 μg/mL, 100.9 ± 8.9 μg/mL, respectively. In angiogenesis, ELESW (100, 500 and 1,000 μg/mL) significantly inhibited VEGF induced angiogenesis as well as VEGF-induced proliferation and tube formation of HUVECs in a dose-dependent manner. Upon stimuli-tion by VEGF, HUVECs rapidly increased reactive oxygen species production, which was significantly blocked by treatment with ELESW. These findings suggesting that S. wightii could be a valuable candidate of therapeutic efficacy