Open AccessSafety evaluation of human peripheral blood mononuclear cells in naive rats:a chronic toxicitystudy
Author(s) -
Basuki Supartono,
Siti Farida,
Sony Suhandono,
Ahmad Aulia Yusuf
Publication year - 2022
Publication title -
bangladesh journal of medical science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.255
H-Index - 10
eISSN - 2079-6854
pISSN - 2076-0299
DOI - 10.3329/bjms.v21i2.57029
Subject(s) - peripheral blood mononuclear cell , medicine , peripheral blood , toxicity , chronic toxicity , transplantation , peripheral , immunology , pharmacology , physiology , in vitro , biochemistry , chemistry
Human peripheral blood mononuclear cells (PBMC) are widely used within tissue engineering therapy, thus, the evaluation of their safety is mandatory. The present study aims to assess the safety of human PBMC transplantations in naive rats in terms of chronic toxicity. Rats received intravenous injections of human PBMC with doses of 105, 106, or 107 cells (n = 5/group/sex) every month for three months without administration of any immunosuppressant drugs. We evaluated clinical, physical, laboratory, and pathology parameters. No morbidity, mortality, or significant differences occurred within all of the tested parameters between control and experimental groups until the finalization of the study. In conclusion, the current work indicates that human PBMC transplantation in naive rats did not induce chronic toxicity reaction.Bangladesh Journal of Medical Science Vol. 21 No. 02 April’22 Page : 373-383