Open AccessAntigenic Cross-Reactivity Between Escherichia albertii DM104 and Different Shigella spp.
Author(s) -
Fatema Moni Chowdhury,
Md. Sakirul Islam Khan,
NilsKåre Birkeland,
Chowdhury Rafiqul Ahsan
Publication year - 2019
Publication title -
bangladesh journal of microbiology
Language(s) - English
Resource type - Journals
eISSN - 2408-8374
pISSN - 1011-9981
DOI - 10.3329/bjm.v35i1.39799
Subject(s) - shigella dysenteriae , shigella flexneri , shigella , microbiology and biotechnology , shigellosis , biology , epitope , antigen , cross reactivity , virology , escherichia coli , serotype , cross reactions , genetics , gene
Environmental Escherichia albertii strain DM104 has been found to induce protective immunity against Shigella dysenteriae in guinea pig model and intranasal immunization showed promising results in terms of antibody response and protective efficacy. For selecting a proper immunodiagnostics marker against shigellosis, the current study investigated the antigenic cross-reactivity between DM104 and four different Shigella spp (S. dysenteriae type 4, S. flexneri type 2a, S. boydii type 15 and S. sonnei). At least six antigenic protein bands (85, 72, 34, 30, 23 and 20 k Da) of the surface components from all these species reacted strongly with both homologous and heterologous antisera, suggesting common distribution of antigenic determinants/epitopes in these bacterial species. This experiment, thus gave a clear idea of the level of antigenic determinants/epitopes sharing and variations between the DM104 and four Shigella spp. Results from this study suggest that the 34, 23 and 20 k Da antigenic proteins may be incorporated as immunodiagnostic marker for the detection of different Shigella spp.
Bangladesh J Microbiol, Volume 35 Number 1 June 2018, pp 17-21