Open AccessRapid micropropagation, antioxidant and antibacterial assays of Ocimum spp.
Author(s) -
Shamoly Akter,
Barna Goswami,
Salim Khan,
Shahina Akter,
Sanjida Rahman Mollika,
Tanjina Akhtar Banu
Publication year - 2020
Publication title -
bangladesh journal of botany
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.152
H-Index - 17
eISSN - 2079-9926
pISSN - 0253-5416
DOI - 10.3329/bjb.v49i3.49332
Subject(s) - ocimum , shoot , dpph , micropropagation , explant culture , botany , murashige and skoog medium , basilicum , chemistry , antibacterial activity , antioxidant , traditional medicine , biology , horticulture , in vitro , bacteria , biochemistry , medicine , genetics
In vitro micropropagation was studied in two species of Ocimum and one species was used for antioxidant and antibacterial activity. The maximum number of shoots/explant (10.1 and 9.5) was induced in MS + 1.0 mg/l BAP + 0.5 mg/l Kn and MS + 2.0 mg/l BAP + 0.5 mg/l IAA in Ocimum tenuiflorum and O. americanum, respectively. GA3 (0.1 mg/l) was found to be effective for shoot induction and elongation in both the species. Strong and stout root formation was observed on half strength MS medium supplemented with 0.2 mg/l IBA for O. tenuiflorum and half strength MS + 0.5 mg/l NAA for O. americanum. Methanolic leaf extract of O. tenuiflorum showed high inhibition of DPPH activity compared to standard antioxidants like quercetin and the chloroform leaf extracts exhibited wide range of antibacterial activity.