Open AccessValidation of neurotoxicological screening methods for chemical substances using reference compounds with differently directed effects on the nervous system
Author(s) -
M.L. Zinovieva,
P.G. Zhminko,
V. V. Kirsenko,
E.N. Strumenska
Publication year - 2019
Publication title -
ukraïnsʹkij žurnal sučasnih problem toksikologìï/ukraïnsʹkij žurnal sučasnih problem toksikologìï
Language(s) - English
Resource type - Journals
eISSN - 2663-9165
pISSN - 2663-4570
DOI - 10.33273/2663-4570-2019-85-1-41-48
Subject(s) - toxicodynamics , chlorpromazine , christian ministry , open field , nervous system , toxicology , pharmacology , caffeine , chemistry , medicine , toxicokinetics , biology , philosophy , theology , psychiatry , pharmacokinetics
Validation of the methods at the stage of their implementation and with subsequent application is an important part of ensuring the quality of toxicological study data State Enterprise “Scientific Center for Preventive Toxicology, Food and Chemical Safety named after Academician L. I. Medved of the Ministry of Health of Ukraine” has implemented methods of screening of neurotoxic exposure of chemical substances — a set of clinical and functional tests (CFT) and a method for evaluation of motor activity in the “open field” for rats.Objective. Determination of specificity, sensitivity and reliability of the implemented methods under exposure to reference neurotropic substances with differently directed effects — chlorpromazine and caffeine on the parameters of the neurological conditions of female Wistar Han rats.Results. It has been shown that the set of CFT and the method for evaluation of motor activity in the “open field” are relevant for the determination of specific exposure and toxicodynamics of reference substances having differently directed effects on the nervous system of rats. The sensitivity of these methods under the lowest active dose of caffeine and chlorpromazine was determined. Data on the reproducibility of test results have been obtained.