Open AccessA Comparative Evaluation and Utilization of Entrapped Bacillus sp. (Gen Bank MN 243657) and Pseudomonas aeruginosa SU-1 in Poultry Waste Degradation
Author(s) -
Lakshmi Srinivasan,
S. Dhiva
Publication year - 2020
Publication title -
proceedings international
Language(s) - English
Resource type - Journals
ISSN - 2668-6384
DOI - 10.33263/proceedings21.072072
Subject(s) - keratinase , biomass (ecology) , pseudomonas aeruginosa , genbank , bacillus (shape) , microbiology and biotechnology , food science , chemistry , bacteria , biology , biochemistry , ecology , genetics , gene
Bacillus sp. (Genbank MN243657) was obtained from the Center for Bioscience and Nanoscience Research, and P. aeruginosa SU-1 were used in this study. Initially, biomass was optimized for the incubation period, pH, and temperature, following biomass optimization with RSM. The obtained organisms were able to produce keratinase, which was found to be a 64 KDa protein and 56KDa. Now the biomass produced using the optimized condition was immobilized and checked for its ability to degrade keratinase, and efficiency was compared. Immobilized P. aeruginosa SU-1 was degrading keratin and feather better than Bacillus sp. (Genbank MN243657). It was found to reduce featherweight by 57.3% and 41%. Hence it was found that both the immobilized bacterium can be used for poultry waste treatment, and P. aeruginosa SU-1 was more efficient than Bacillus sp. (Genbank MN243657).