Open AccessPreparation of Chitosan from Crab Shell and its Antibacterial Activity Against UTI Pathogens
Author(s) -
A. Mini Varsini,
S. Abirami,
P Revathi,
S Sakthikavitha
Publication year - 2020
Publication title -
proceedings international
Language(s) - English
Resource type - Journals
ISSN - 2668-6384
DOI - 10.33263/proceedings21.014014
Subject(s) - chitosan , chitin , proteus , chemistry , antibacterial activity , escherichia coli , yield (engineering) , microbiology and biotechnology , acetylation , food science , shell (structure) , proteus vulgaris , bacteria , nuclear chemistry , biology , biochemistry , materials science , genetics , metallurgy , composite material , gene
Chitosan was extracted from the chitin of crab shells. The process of deacetylation removes acetyl groups from the molecular chain of chitin. The yield of chitosan was 37.5%, moisture content was 64.32%, water binding capacity was 58.44%. This study concluded that the FT-IR, NMR method is one of the best methods of determination of deacetylation. The crab shell extracted chitosan had significantly inhibited the pathogenic Escherichia coli, Proteus sp., and Klebsiella sp., isolated from UTI infected patients.