Open AccessA study on optimization of biomass of Bacillus pumilus for feather degradation
Author(s) -
S. Dhiva
Publication year - 2020
Publication title -
proceedings international
Language(s) - English
Resource type - Journals
ISSN - 2668-6384
DOI - 10.33263/proceedings21.005005
Subject(s) - bacillus pumilus , keratinase , agar , feather , bacteria , biomass (ecology) , agar plate , bacillus (shape) , food science , incubation , pellet , biology , microbiology and biotechnology , degradation (telecommunications) , starch , nutrient agar , incubation period , chemistry , agronomy , biochemistry , ecology , telecommunications , genetics , computer science
Soil samples were collected from the feather dumped area, and they were screened for the presence of keratinolytic bacteria Bacillus pumilus. Based on its growth on Bacillus isolation agar, Skim milk agar, and Starch agar, it was conformed as Bacillus pumilus. The growth of bacteria was estimated by biomass estimation. In the optimization study, the optimum incubation period observed for feather degradation was 48hrs, pH 7, and temperature 40°C. Purified Keratinase enzyme was used for the feather degradation study. The maximum degradation observed was 29% at the temperature of 400C. The size of kerinase produced was estimated as 52KDa.