Immobilization of lignin peroxidase from Alcaligenes aquatilis and its application in dye decolorization | Zendy

Shawky Sabae | Zendy; Ayman Eldourghamy | Zendy; Samar A. Aly | Zendy; Nashwa Rizk | Zendy; Ahmed Sobhy | Zendy; C Hsueh | Zendy; B Chen | Zendy; R Saratale | Zendy; G Saratale | Zendy; J Chang | Zendy; S Govindwar | Zendy; K Pavithra | Zendy; P Kumar | Zendy; V Jaikumar | Zendy; P Rajan | Zendy; N Daneshvar | Zendy; M Ayazloo | Zendy; A Khataee | Zendy; M Pourhassan | Zendy; S Asad | Zendy; M Amoozegar | Zendy; A Pourbabaee | Zendy; M Sarbolouki | Zendy; S Dastghei | Zendy; Dos Santos | Zendy; A Cervantes | Zendy; F J; Van Lier | Zendy; J | Zendy; T Robinson | Zendy; B Chandran | Zendy; P Nigam | Zendy; N Nadir | Zendy; N Ismail | Zendy; A Hussain | Zendy; A Kumar | Zendy; R Chandra | Zendy; E Erden | Zendy; C Ucar | Zendy; T Gezer | Zendy; N Pazarlioglu | Zendy; R Sheldon | Zendy; C Reis | Zendy; E Sousa | Zendy; J Serpa | Zendy; R Oliveira | Zendy; J Santos | Zendy; K Kaur | Zendy; P Kaushal | Zendy; B Varga | Zendy; M Meiczinger | Zendy; V Somogyi | Zendy; S Ghada | Zendy; H Amany | Zendy; Y Zhou | Zendy; L Wang | Zendy; T Wu | Zendy; X Tang | Zendy; S Pan | Zendy; H Ogola | Zendy; T Kamiike | Zendy; N Hashimoto | Zendy; H Ashida | Zendy; T Ishikawa | Zendy; H Shibata | Zendy; Y Sawa | Zendy; O Lowry | Zendy; N Rosbrough | Zendy; A Farr | Zendy; R Randall | Zendy; S Kumar | Zendy; I Haq | Zendy; A Yadav | Zendy; J Prakash | Zendy; A Raj | Zendy; D Yanto | Zendy; S Tachibana | Zendy; K Itoh | Zendy; H Qiu | Zendy; Y Li | Zendy; G Ji | Zendy; G Zhou | Zendy; X Huang | Zendy; Y Qu | Zendy; P Gao | Zendy; S Oliveira | Zendy; J Da Luz | Zendy; M Kasuya | Zendy; L Ladeira | Zendy; A Junior | Zendy; M Chang | Zendy; R Juang | Zendy; A Basso | Zendy; S Serban | Zendy; H Rehman | Zendy; A Aman | Zendy; A Silipo | Zendy; S Qader | Zendy; A Molinaro | Zendy; S Talekar | Zendy; S Chavare | Zendy; P Sofia | Zendy; M Asgher | Zendy; M Shahid | Zendy; M Randhawa | Zendy; A Dwevedi | Zendy; A Kayastha | Zendy; A Modenez | Zendy; D Sastre | Zendy; C Moraes | Zendy; C Marques Netto | Zendy; H Mageed | Zendy; N Ezz | Zendy; R Radwan | Zendy; H El-Shora | Zendy; M Ibrahim | Zendy; M Elmekabaty | Zendy; B Jiang | Zendy; Y Zhang | Zendy; R Georgiou | Zendy; E Tsiakiri | Zendy; N Lazaridis | Zendy; A Pantazaki | Zendy; S Romo-Sanchez | Zendy; C Camacho | Zendy; H Ramirez | Zendy; M Arevalo-Villena | Zendy; T Nwagu | Zendy; H Aoyagi | Zendy; B Okolo | Zendy; S Yoshida | Zendy; F Zheng | Zendy; B Cui | Zendy; X Wu | Zendy; G Meng | Zendy; H Liu | Zendy; J Si | Zendy; M Bilal | Zendy; Z Jing | Zendy; Y Zhao | Zendy; H Iqbal | Zendy; F Hasegawa | Zendy; H Inoue | Zendy; S Yano | Zendy; S Yokoyama | Zendy; K Imou | Zendy; C Nicolucci | Zendy; S Rossi | Zendy; C Menale | Zendy; T Godjevargova | Zendy; Y Ivanov | Zendy; M Bianco | Zendy; L Mita | Zendy; U Bencivenga | Zendy; D Mita | Zendy; N Diano | Zendy; R Pang | Zendy; M Li | Zendy; C Zhang | Zendy; U Sneha | Zendy; R Poornima | Zendy; S Sridhar | Zendy; Z Hu | Zendy; ; Xu | Zendy; X Wen | Zendy

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Immobilization of lignin peroxidase from Alcaligenes aquatilis and its application in dye decolorization

Author(s) -

Shawky Sabae,

Ayman Eldourghamy,

Samar A. Aly,

Nashwa Rizk,

Ahmed Sobhy,

C Hsueh,

B Chen,

R Saratale,

G Saratale,

J Chang,

S Govindwar,

K Pavithra,

P Kumar,

V Jaikumar,

P Rajan,

N Daneshvar,

M Ayazloo,

A Khataee,

M Pourhassan,

S Asad,

M Amoozegar,

A Pourbabaee,

M Sarbolouki,

S Dastghei,

Dos Santos,

A Cervantes,

F J; Van Lier,

J,

T Robinson,

B Chandran,

P Nigam,

H Iqbal,

M Asgher,

N Nadir,

N Ismail,

A Hussain,

A Kumar,

R Chandra,

E Erden,

C Ucar,

T Gezer,

N Pazarlioglu,

R Sheldon,

C Reis,

E Sousa,

J Serpa,

R Oliveira,

J Santos,

K Kaur,

P Kaushal,

B Varga,

M Meiczinger,

V Somogyi,

H El-Shora,

S Ghada,

H Amany,

Y Zhou,

L Wang,

T Wu,

X Tang,

S Pan,

H Ogola,

T Kamiike,

N Hashimoto,

H Ashida,

T Ishikawa,

H Shibata,

Y Sawa,

O Lowry,

N Rosbrough,

A Farr,

R Randall,

S Kumar,

I Haq,

A Yadav,

J Prakash,

A Raj,

D Yanto,

S Tachibana,

K Itoh,

H Qiu,

Y Li,

G Ji,

G Zhou,

X Huang,

Y Qu,

P Gao,

S Oliveira,

J Da Luz,

M Kasuya,

L Ladeira,

A Junior,

M Chang,

R Juang,

A Basso,

S Serban,

H Rehman,

A Aman,

A Silipo,

S Qader,

A Molinaro,

S Talekar,

S Chavare,

P Sofia,

M Asgher,

M Shahid,

M Randhawa,

A Dwevedi,

A Kayastha,

A Modenez,

D Sastre,

C Moraes,

C Marques Netto,

H Mageed,

N Ezz,

R Radwan,

H El-Shora,

M Ibrahim,

M Elmekabaty,

B Jiang,

Y Zhang,

R Georgiou,

E Tsiakiri,

N Lazaridis,

A Pantazaki,

S Romo-Sanchez,

C Camacho,

H Ramirez,

M Arevalo-Villena,

T Nwagu,

H Aoyagi,

B Okolo,

S Yoshida,

F Zheng,

B Cui,

X Wu,

G Meng,

H Liu,

J Si,

M Bilal,

Z Jing,

Y Zhao,

H Iqbal,

F Hasegawa,

H Inoue,

S Yano,

S Yokoyama,

K Imou,

C Nicolucci,

S Rossi,

C Menale,

T Godjevargova,

Y Ivanov,

M Bianco,

L Mita,

U Bencivenga,

D Mita,

N Diano,

R Pang,

M Li,

C Zhang,

U Sneha,

R Poornima,

S Sridhar,

Z Hu,

; Xu,

X Wen

Publication year - 2020

Publication title -

letters in applied nanobioscience

Language(s) - English

Resource type - Journals

ISSN - 2284-6808

DOI - 10.33263/lianbs92.10581063

Subject(s) - lignin peroxidase , chemistry , lignin , adsorption , thermostability , malachite green , peroxidase , immobilized enzyme , nuclear chemistry , thermal stability , horseradish peroxidase , chitosan , chromatography , organic chemistry , enzyme

Purefied lignin peroxidase produced from Alcaligenes aquatilis DB8 was immobilized by entrapment on Ca-alginate and chitosan by adsorption and cross linker. Immobilization yields of entrapment, adsorption and cross linking methods were 79.6%, 31.4% and 91.2%, respectively. The thermostability of immobilized LiP by entrapment, adsorption and crosslinking retained residual activity of 68%, 63% and 85%, respectively after 180 min at 50 °C. While the residual activity of free enzyme was decreased to almost half after 180 min. Lignin peroxidase immobilized by crosslinking showed high thermal stability, more than 70% of the enzymatic activity remained after 240 min. Also, immobilized lignin peroxidase by crosslinking showed better storage stability about 55% at 30 days. The application of immobilized lignin peroxidase in a packed bead bioreactor enhanced decolorization of malachite green at 20 mg/l, and the percent of decolorization was 97% at 4 h.

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