Open AccessHPLC method development for estimation of pitavastatin in liquid dosage form
Author(s) -
Shubham Verma,
Nitin Kumar,
Pawan Kumar,
Gaurav K. Jain
Publication year - 2020
Publication title -
letters in applied nanobioscience
Language(s) - English
Resource type - Journals
ISSN - 2284-6808
DOI - 10.33263/lianbs92.10001003
Subject(s) - chromatography , dosage form , pitavastatin , high performance liquid chromatography , calibration curve , correlation coefficient , chemistry , particle size , reproducibility , materials science , analytical chemistry (journal) , detection limit , biochemistry , statin , statistics , mathematics
For the determination of pitavastatin calcium nanoemulsion liquid dosage form a simple, sensitive, reliable and rapid reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated. 5 μ particle size column in isocratic mode at 25ºC temperature. The sample was injected through an injector valve with a 10 μl, sample loop. Phosphate buffer (pH 6.4): Methanol (50:50 v/v) was used as a mobile phase with a flow rate of 1 ml per min at 286 nm wavelength. A calibration graph was plotted range between 25-200 μg/ml with the correlation coefficient of 0.999 which showed linearity. Validation studies revealed the method is specific, rapid, reliable, and reproducible. The validity of the method, degradation studies were carried out using the same optimum conditions. Therefore the proposed method is reliable, rapid, precise and selective and may be used for the quantitative analysis of nanoemulsion liquid dosage form of pitavastatin calcium.