Open AccessSpectroscopic Fourier Transform Infrared Study of Poly (N-Isopropylacrylamide)-Human Recombinant Platelet-Derived Growth Factor- BB Protein as a Strategic Biobetter Regenerative Material
Publication year - 2021
Publication title -
letters in applied nanobioscience
Language(s) - English
Resource type - Journals
ISSN - 2284-6808
DOI - 10.33263/lianbs113.39273933
Subject(s) - recombinant dna , platelet derived growth factor receptor , in vitro , chemistry , growth factor , fourier transform infrared spectroscopy , platelet derived growth factor , fibroblast , microbiology and biotechnology , materials science , biochemistry , biology , chemical engineering , receptor , engineering , gene
Biobetter or Biosuperior are recombinant protein drugs with one or more characteristics that are better than the original formulation. Platelet-Derived Growth Factor (PDGF) is a potent mitogen that can induce pulp stem cell proliferation and promote angiogenesis in vitro. PDGF-BB is required for wound healing, and they are chemoattractants that can activate macrophages and fibroblast activation in vitro. Poly(n-isopropylacrylamide) (PNIPAM) polymer is a thermoresponsive polymer widely used for drug delivery. A lyophilized combination of PNIPAM and 5µg of Recombinant Human PDGF-BB(rhPDGF-BB)(sigma Aldrich-P3201) protein was prepared in the present study. This combination was subjected to Spectroscopic Fourier-Transform Infrared(FTIR) study. The results suggested that a biophysical approach like FTIR enables assessing protein stability and aggregation tendency of recombinant proteins, contributing as a useful method to identify samples with prospective high therapeutic values.