Open AccessSynthesis and Characterization of Gold Nanoparticles from Nostoc sp. strain HKAR-2 and their In Vitro Antibacterial, Antifungal, and Antitumor Potentials
Author(s) -
Arun S. Sonker,
Jainendra Pathak,
Rajeshwar P. Sinha
Publication year - 2021
Publication title -
letters in applied nanobioscience
Language(s) - English
Resource type - Journals
ISSN - 2284-6808
DOI - 10.33263/lianbs111.30203035
Subject(s) - colloidal gold , nuclear chemistry , selected area diffraction , fourier transform infrared spectroscopy , absorbance , surface plasmon resonance , zeta potential , scanning electron microscope , transmission electron microscopy , nanoparticle , materials science , chemistry , nanotechnology , chromatography , chemical engineering , engineering , composite material
In the present study, gold nanoparticles (AuNPs) have been synthesized through biological methods using the cell-free extracts of cyanobacterium Nostoc sp. strain HKAR-2 isolated from the hot spring of India. To determine morphological, structural, and optical properties of green synthesized AuNPs, UV-VIS spectroscopy, FTIR, X-ray diffraction, scanning electron microscopy (SEM), and transmission electron microscopy-selected area electron diffraction (TEM-SAED) were used. Spectroscopic analysis showed the absorbance peak at 540 nm due to the reduction of Au3+ to Au0 by cyanobacterial extract, which indicated surface plasmon resonance (SPR) of the synthesized AuNPs. Characteristic Bragg peaks at (111), (200), (220), and (311) facets of the face center cubic (fcc) confirmed the crystalline nature of AuNPs in the XRD pattern. FTIR results indicated the role of proteins and amino acids in the reduction of Au3+ to Au0 as well as for the stability of AuNPs. Zeta potential confirmed a charge of -2.39Mv on the AuNPs. SEM and TEM results confirmed the large agglomerated shape of AuNPs with sizes ranging between 10-100 nm. Their antibacterial, antifungal and anticancerous properties were also studied against plant bacterial strains, fungal strains, and MCF-7 cells, respectively. AuNPs also showed dose-dependent cytotoxic activity against human breast cancer MCF-7 cells with IC50 of 250 μg/mL.