Lactobacillus spp. Inhibit the Biofilm Formation of Pseudomonas aeruginosa

Probiotic bacteria are microbial food supplements with beneficial properties on human health. This study aimed to isolate Lactobacillus spp. from the traditional homemade dairy and evaluate their inhibitory effects on the biofilm formation of P. aeruginosa. In this study, 10 samples of traditional homemade yogurt and milk samples were collected from five separated rural areas of Miyana, Azerbaijan, Iran, from May-June 2016. Lactobacillus spp. was isolated from traditional homemade dairy using microbiological methods. Finally, Lactobacillus spp. effects on P. aeruginosa biofilm were determined by the modified micro-plate test. Fifty-eight acid-tolerance bacteria were isolated from milk and yogurt samples. L. plantarum and L. casei had antibiofilm activity. The percentage of biofilm inhibition by L. plantarum and L. casei isolated from yogurt and milk were obtained zero to 52.1% and 5.7% to 48.8%; and zero to 25.8% and 5.2% to 16.1%, respectively. L. plantarum and L. casei isolated from traditional milk and yogurt have an inhibition effect on the biofilm formation of P. aeruginosa. It suggests the traditional homemade dairy is one of the best probiotics against biofilm-producing P. aeruginosa.