Open AccessDevelopment and Validation of a Fast and Selective HPLC Method for the Determination of Amphotericin B in Nose-to-Brain Nanoliposome
Author(s) -
Alessandra Cândida Rios,
Fernando Batain,
Cecília de Lima Barros,
Daniela De Paula,
M. R. F. B. Martins,
Marco V. Chaud,
Thaís Francine Ribeiro Alves
Publication year - 2020
Publication title -
letters in applied nanobioscience
Language(s) - English
Resource type - Journals
ISSN - 2284-6808
DOI - 10.33263/lianbs102.23092319
Subject(s) - chromatography , high performance liquid chromatography , methanol , acetonitrile , amphotericin b , relative standard deviation , chemistry , particle size , tetrahydrofuran , volumetric flow rate , analytical chemistry (journal) , materials science , detection limit , antifungal , medicine , organic chemistry , solvent , physics , quantum mechanics , dermatology
A simple, fast, and selective HPLC method is presented to quantified amphotericin B (AmB) in a nose-to-brain nanoliposomal pharmaceutical formulation. The development was based on the design of experiments (DoE) approach. The chromatographic analysis was validated on a C18 Zorbax reversed-phase column (250 mm x 4,6 mm I.D.) with 5 µm of particle size using mobile phase, consisting of a binary mixture of ultra-purified water and an organic composition of acetonitrile, methanol, and tetrahydrofuran (75:17:8, v/v). The isocratic flow rate was 1.0 mL.min-1, and the detection at 383 nm. The 12 minutes running time being selective between the main peak from possible degradation products, linear and accurate for the concentration range of 0.5 to 7.0 μg.mL-1, and precise demonstrating a relative standard deviation of 0.01 % (n = 6). Application of this method to assay and stability studies of AmB in nanoliposomal lipid-based is provided.