Open AccessProteomic Profiling of Human Umbilical Cord Blood Serum and its Potential Use in Regenerative Therapies
Publication year - 2020
Publication title -
letters in applied nanobioscience
Language(s) - English
Resource type - Journals
ISSN - 2284-6808
DOI - 10.33263/lianbs102.21672173
Subject(s) - umbilical cord , biology , vacutainer , proteomics , blood proteins , fetus , proteome , embryo , bioinformatics , computational biology , microbiology and biotechnology , immunology , medicine , gene , pregnancy , biochemistry , genetics
Application of mediators directly to the injured site to promote de novo tissue can be the most promising strategy in regenerative medicine. However, understanding the mechanism of regional application of proteins /growth factors is challenging. Likewise, the proteins from Human Umbilical Cord Blood Serum(hUCBS) remain inadequately understood in terms of its therapeutic use. Several biological processes of the components in hUCBS remains unanswered. Human umbilical cord blood was collected in vacutainer tubes from 35 healthy mothers.hUCBS was obtained after centrifuge -4oC at 10,000 rpm for 20minutes. After the removal of high abundant proteins, proteomic profiling of UCBS was done by Mass Spectrometry. The Mascot search engine was used for peptide sequence identification. Proteins identified were assessed for tissue expression, cellular components, and biological functions based on Gene Ontology (GO) analyses. A total of 99 proteins were detected. The tissue expression information of proteins obtained included the Stomach, Liver, Heart, Lungs. Around 91 proteins were expressed in embryo organs. Proteins in hUBCS reflect the physiological status of the fetus/pregnancy. Major proteins found in hUCBS were expressed in the embryo, indicating that proteins were involved in organ development. Data contributed to a better understanding of the protein functions present in hUCBS.