Open AccessSeparation of D,L-Ampicillin by ligand exchange-micellar electrokinetic chromatography
Author(s) -
Duygu Sarı,
Ali Derazshamshir,
Süleyman Aşır,
İlgım Göktürk,
Fatma Yılmaz,
Adi̇l Deni̇zli̇
Publication year - 2019
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac96.522533
Subject(s) - chemistry , micellar electrokinetic chromatography , micelle , chromatography , capillary electrophoresis , aqueous solution , electrokinetic phenomena , detection limit , electrophoresis , ligand (biochemistry) , organic chemistry , biochemistry , receptor
In this study, D,L-ampicillin separation was carried out by ligand exchange-micellar electrokinetic chromatography method using L-Lysine monohydrochloride as a ligand and copper (II) sulfate pentahydrate is a central ion supplier. Isomeric separations were performed using capillary electrophoresis (CE) instrument, in which SDS-L-Lys-Cu+2 micelle complex was used as a pseudostationary phase. The effect of pH, SDS amount, applied electrical field, pressure, organic solvent ratio and ampicillin D,L-ratios were investigated. Fast, inexpensive and sensitive approach for the simultaneous separation of D,L-ampicillin in both aqueous and real antibiotic sample was performed using CE coupled with UV detector. The separation was achieved in a short period of 7 minutes with high-sensitivity and low-detection limit of 1.25 μM by the developed SDS-L-Lys-Cu+2 micelle-chiral selector complexes without using any extra process such as imprinting or spacer arms for the immobilization of the ligands.