Open AccessAnalytical procedures and methods validation for oxalate content estimation
Author(s) -
Dina Karamad,
Kianoush KhosraviDarani,
Hedayat Hosseini,
Sanaz Tavasoli
Publication year - 2019
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac95.305310
Subject(s) - oxalate , calcium oxalate , chemistry , kidney stones , titration , potassium , urinary system , chromatography , inorganic chemistry , medicine , organic chemistry
Increased urinary oxalate is considered a major risk factor in the formation of calcium oxalate kidney stones. Gut microbiota may reduce the risk of stone formation. Anyway, the first step for any research about monitoring of oxalate content (both in vitro and in vivo) is a determination of its concentration, while there are different methods reported in the literature for oxalate content determination. In this research, the main reported methods including titration with two titrators (potassium permanganate, and NaOH) as well as enzymatic method (oxalate assay kit) are presented and compared for the measurement of oxalate in both inoculated and non-inoculated media.