Open AccessPhenotypical studies of raw and nanosystem embedded Eugenia carryophyllata buds essential oil antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus strains
Author(s) -
Crina Saviuc,
Alexandru Mihai Grumezescu,
Alina Maria Holban,
Coralia Bleoţu,
Mariana Carmen Chifiriuc,
Paul Cătălin Balaure,
Veronica Lazăr
Publication year - 2011
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac13.111118
Subject(s) - essential oil , pseudomonas aeruginosa , antimicrobial , staphylococcus aureus , in vivo , microbiology and biotechnology , hela , minimum inhibitory concentration , chemistry , antibacterial activity , virulence , biology , in vitro , bacteria , food science , biochemistry , gene , genetics
Considering the large number of the multiresistant bacterial strains and the increasing need in new antimicrobial formulation, the aim of this study was to evaluate the antipathogenic effect of E. caryophyllata essential oil on collection and recently isolated clinical strains of P. aeruginosa and S. aureus. Experimental assays were carried out by in vitro and in vivo tests. Qualitative and quantitative assessment of the antimicrobial activity was followed by the analysis of the essential oil influence on enzymatic soluble virulencefactors expression. The cytotoxic effect of the vegetal extract was quantified by usingHeLa cells. Nanosystem embedded essential oil was used to establish the in vivoantipathogenic effect on mice. Qualitative screening results revealed an early microbicidaleffect quantified by low minimum inhibitory concentration values. The in vivo studyshowed a stabiliziation of the essential oil biological activities when using nanosystemembedding, that could be used for the design of proper formulations for delivery systemswith antimicrobial effect.