Open AccessBioactive Polysaccharides Based Graphene Oxide Nanoparticle as a Promising Carrier for Anticancer Drug Delivery
Author(s) -
Sonia Kesavan,
Karunakaran Sulochana Meena,
Rajesh Dhakshinamoorthy
Publication year - 2021
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac123.34293445
Subject(s) - nanocarriers , chitosan , graphene , biocompatibility , drug delivery , fourier transform infrared spectroscopy , materials science , nanotechnology , mtt assay , nanoparticle , nanomedicine , nuclear chemistry , raman spectroscopy , oxide , chemistry , xyloglucan , chemical engineering , polysaccharide , organic chemistry , in vitro , biochemistry , physics , optics , engineering
Nowadays, the concept of drug transmission is a prominent issue in the world of drug delivery research. We investigated the development of a hybrid platform based on graphene oxide/chitosan and xyloglucan (GO-CH-Xn) for the loading and release of doxorubicin (DOX)., where chitosan (CS) natural polymer functionalizes graphene oxide and is then grafted by xyloglucan (Xn) natural hydrophilic polysaccharide to form a reliable nanocarrier system for the delivery of DOX. UV-Vis spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, Raman spectroscopy, transmission electron microscopy, and scanning electron microscopy analysis were used to confirm the fundamental physicochemical properties. The DOX loading capacity and efficiency were 81.8% and 73.5%. The graphene oxide-chitosan-xyloglucan- doxorubicin (GO-CS-Xn-DOX) drug delivery system showed a pH-regulated release as observed by UV analysis. Biocompatibility was evaluated via in vitro hemolysis assay, indicates negligible toxicity, and the anticancer activity of the developed nanocarrier system was studied by 3-(4, 5-dimethylthiazol-2-Y)-2,5-diphenyltetrazolium bromide (MTT) against human (U 87) glioblastoma cancer cell lines. The in vitro studies demonstrate the major advantages of the developed approach by demonstrating its capability as a promising nanocarrier for biomedical applications.