Open AccessAn Eco-Concerned Development of a Fast, Precise and Economical Spectrophotometric Assay for the Antiviral Drug Simeprevir based on Ion-Pair Formation
Author(s) -
Sabrein H. Mohamed,
Yousry M. Issa,
Alyaa I. Salim
Publication year - 2021
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac115.1347413489
Subject(s) - bromothymol blue , bromocresol green , chemistry , detection limit , bromocresol purple , chromatography , ion association , molar absorptivity , mass spectrometry , simeprevir , analytical chemistry (journal) , ion , organic chemistry , physics , optics , biochemistry , ribavirin , genotype , gene
Simeprevir sodium (SMV) is one of the antiviral drugs used for the treatment of virus C. The current strategy develops and validates a new eco-concerned tool for its quantification in the pure and pharmaceutical formulations. Sulfonephthalein acid dyes were used for this purpose, applying visible analyses based on ion-pair formation. A linear relation between the absorbed signal and the drug concentration is obtained up to 67.0 μg mL-1 with r2 of 0.9989-0.9999. The measurement is carried out at 410, 415, 410, and 403 nm for bromocresol green, bromoxelenol blue, bromothymol blue, and bromocresol purple, respectively, in dichloromethane as a solvent. The drug structure was confirmed utilizing different tools; mass spectrometry, FT-IR, 1H NMR, and thermal analysis. Association, formation constants, molar absorptivity, and free energy change for SMV-ion-pairs were calculated. The limit of detection reaches 50.0 ng mL-1 with a quantification limit of 180.0 ng mL-1. The recovery values are 96.44-104.39%, with a relative standard deviation 0.15-1.37%. For confirmation of the obtained results, they were statistically compared with a previously published HPLC method utilizing t- and F- tests.