Open AccessGC/MS and HPTLC-based Methods of Comparison among Standard and Different Commercial Samples of Ferula gummosa Boiss
Author(s) -
Boiss Moein,
Saeed Maalhagh-Fard,
Atefeh Jalali,
Sepideh Habibzadeh,
Mohammad M. Zarshenas
Publication year - 2020
Publication title -
biointerface research in applied chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.216
H-Index - 11
ISSN - 2069-5837
DOI - 10.33263/briac114.1107711087
Subject(s) - dichloromethane , chromatography , gas chromatography–mass spectrometry , chemistry , gas chromatography , essential oil , quality standard , traditional medicine , mass spectrometry , organic chemistry , medicine , solvent
Ferula gummosa (Apiaceae) Boiss. as a valuable herbal medicine possesses various medical and industrial applications. The oleo-gum resin of F. gummosa, called Galbanum, holds several biological activities for its numerous terpenoid compounds. This study has been conducted on quality control of the F. gummosa oleo-gum resin prepared from standard plants, and commercial samples belong to different parts of Iran. For this purpose, essential oil and dichloromethane extracts and standard fruit essential oil were obtained and evaluated by Gas Chromatography/Mass Spectrometry (GC/MS). Moreover, all dichloromethane oleo-gum resin samples (Standards and commercials) were qualitatively analyzed by High-Performance Thin-Layer Chromatography (HPTLC). Based on the GC/MS analysis, β-Pinene, δ-3-Carene and, α-Pinene in standard and β-Pinene, α-Pinene and, δ-3-Carene in essential oil, commercial samples were recognized as major compounds, respectively. The GC/MS analysis indicates that all commercial oleo-gum resin samples may obtain from the rhizome except one of them. The HPTLC analysis also revealed that the same spot pattern in all samples might be related to the major resin constituents. However, the source of oleo-gum resin could not be clarifying. Based on the results, both GC/FID and HPTLC analysis are useful methods for quality control of oleo-gum resin. The plant part used for oleo-resin extraction can be recognized via the percentage of essential compounds in it.