Bio-manufacturing of selenium nanoparticles by Bacillus subtilis isolated from Qarun Lake and evaluation their activity for water remediation

Selenium nanoparticles (Se-NPS) have gently stimulated more extensive interest due to their vital properties. During this intensive investigation, we have to tend to restrict aquatic bacteria isolated from Qarun Lake for the inexperienced green synthesis of nanoselenium and its potential applications as antimicrobial for water purification. Twenty-four distinctive bacterial isolates were purified and screened for selenite resistance, and ten were positive. Out of them, isolate of Bats2 had been excellently tolerant of sodium selenite with minimal inhibitory concentrations (MIC) 18 g/l. They have continually been the best isolate in the manufacture of Se-NPS with maximal coloration and absorbance at 420 nm, in 3 days growth at pH 7 and 35 °C with 1g/l sodium selenite. The strongest extracellular biomanufacturing of Se-NPS has been properly identified as Bacillus subtilis. The facile fabricated Se-NPS by the acceptable and most powerful isolate Bacillus subtilis have been consistently characterized using UV-visible spectroscopy, XRD, SEM and TEM techniques. Biosynthesized Nanoparticles had diameter of 31-193 nm using and the typical XRD patterns sufficiently established the imprecise nature of the fabricated nanoparticles. The significant impact of Se-NPs was fastidiously observed on the potential growth of pathogenic Gram-positive and Gram-negative bacteria. Our desired outcomes absolutely confirmed the antimicrobial activity of Se-NPs only against Staphylococcus aureus. Thus, bacterial Se-NPs composite might realize appropriate application as a bioremediation tool of pathogens in required water.